Dating bloodstains with fluorescence lifetime measurements.

Either your web browser doesn’t support Javascript or it is currently turned off. In the latter case, please turn on Javascript support in your web browser and reload this page. Free to read. In May , a young woman was found severely injured in the Eltham district of London, her head brutally smashed with a plaster’s hammer. One of the wounds divided the temporal artery, resulting in a large discharge of blood. Several days later, a year old man, her friend and alleged lover, was arrested. Numerous small spots of blood were found on his shirt. The examiner of the case, Dr. Letheby, one of the pioneers of forensic spectral analysis of blood, declined to assign a date to the bloodstains on the shirt and connect the defendant to the murder. This statement outweighed the other evidence, the man was sent free, and the crime known as the Eltham murder remains a mystery.

Amino acid residues give away bloodstain’s age

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Estimation of the age of human bloodstains is of great importance in forensic practices, but it is a challenging task because of the lack of a well-accepted, reliable, and established method.

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WO2009130580A1 – Apparatus and method for dating a body sample – Google Patents

The present invention relates to an apparatus and method for dating a body sample, preferably, but not exclusively, a sample of body fluid, such as blood, which may be external to the body, for example, having caused stains on an external substrate, such as a floor, wall, etc. It is well known that dating of both external blood stains and internal bruising is a useful forensic technique to determine when a particular injury was caused to a person.

In the one case, the injury would have been such that the skin was punctured and blood was spilt and in the other case no blood was spilt externally, but bruising, to a greater or lesser extent was caused.

Dating bloodstains with fluorescence lifetime measurements. On Measuring the Volume of very small Drops of Blood and Correlation of this.

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Guo, K., Achilefu, S. and Berezin, M.Y. () Dating bloodstains with fluorescence lifetime measurements. Chemistry, – Hanson, E.K. and.

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Publications by authors named “Kevin Guo”

Studies of inactivation mechanism of non-enveloped icosahedral virus by a visible ultrashort pulsed laser. Virology journal. Automatic segmentation of fluorescence lifetime microscopy images of cells using multiresolution community detection–a first study. Journal of microscopy. Probing distance-dependent plasmon-enhanced near-infrared fluorescence using polyelectrolyte multilayers as dielectric spacers.

Angew Chem Int Ed Engl.

bloodstains dating studies, as it should give an insight into formation of different Hb solid state fluorescent lifetime measurements, Proc.

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Optical Radiology Laboratory (ORL)

Raman spectroscopy and advanced statistics allow the researchers to date a blood stain accurately, provided said blood stain is less than two years old. Raman spectroscopy involves shining a laser on a sample and measuring the intensity of scattered light. No two substances give the exact same Raman spectrum and so the spectrum is essentially a unique fingerprint for a given material.

Creamer, J.I., et al., Attempted cleaning of bloodstains and its effect on the and M.Y. Berezin, Dating bloodstains with fluorescence lifetime measurements.

This application claims the benefit of U. Provisional Application No. The field of the disclosure relates generally to dating biological samples through the use of fluorescence. Throughout history, bloodstain dating has played a significant role in areas such as criminal justice. Numerous violent crimes have remained unsolved for lack of evidence and the incapability to assign timing of bloodstains and other bodily fluids, thereby placing victims and potential defendants at the scene of the crime during the period at issue.

Particularly, in the last two hundred years, criminal investigators explored a variety of approaches to establish a reliable quantitative method, from the evaluation of bloodstain color as shown in total organic carbon TOC analysis, microscopic analysis of red corpuscles and a water solubility scale in the middle of the 19th century, to electrochemistry, electron paramagnetic resonance, atomic force microscopy, polymer chain reaction, and optical spectroscopy in the 21 st century.

CSI: The Age of the Bloodstain

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Date of publication April 16, ; date of current age of bloodstains by enzyme activities in blood cells in [4] Using ratios of S. Mc Shine et al. applied fluorescence lifetime to determine the time since the plane mirrors and finally focused on the sample surface by a convex lens with a focal length of. mm.

Lydia Sauer, Christopher B. Komanski, Alexandra S. Vitale, Eric D. Hansen, Paul S. Fluorescence decays were detected in two spectral channels — nm; — nm to determine fluorescence lifetimes of endogenous fluorophores in their specific spectral emission ranges. Blood samples from a healthy subject and a patient with PED were investigated in a quartz cuvette. Additionally, ex vivo studies of human blood help to better interpret FLIO images.

Purchase this article with an account. Jump To Lydia Sauer ; Christopher B. Komanski ; Alexandra S. Vitale ; Eric D.

Lifetime Concept